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pact2 hela cell cdna library  (TaKaRa)


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    TaKaRa pact2 hela cell cdna library
    YFV NS5 (RNApol)- human eIF3L interaction in a yeast two - hybrid system. Yeast strain AH109 was co-transformed with pGBKT7-RNApol (or the empty or Lamin C human protein BD vector) and <t>pACT2-eIF3L</t> (or the empty or SV40 large T-antigen AD vector). The specific interaction was identified by the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE) with the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.
    Pact2 Hela Cell Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pact2+hela+cell+cdna+library/Human+Kidney+Matchmaker+cDNA+Library+pACT2/pmc03698205-138-6-11
    Average 93 stars, based on 157 article reviews
    pact2 hela cell cdna library - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "The eukaryotic translation initiation factor 3 subunit L protein interacts with Flavivirus NS5 and may modulate yellow fever virus replication"

    Article Title: The eukaryotic translation initiation factor 3 subunit L protein interacts with Flavivirus NS5 and may modulate yellow fever virus replication

    Journal: Virology Journal

    doi: 10.1186/1743-422X-10-205

    YFV NS5 (RNApol)- human eIF3L interaction in a yeast two - hybrid system. Yeast strain AH109 was co-transformed with pGBKT7-RNApol (or the empty or Lamin C human protein BD vector) and pACT2-eIF3L (or the empty or SV40 large T-antigen AD vector). The specific interaction was identified by the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE) with the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.
    Figure Legend Snippet: YFV NS5 (RNApol)- human eIF3L interaction in a yeast two - hybrid system. Yeast strain AH109 was co-transformed with pGBKT7-RNApol (or the empty or Lamin C human protein BD vector) and pACT2-eIF3L (or the empty or SV40 large T-antigen AD vector). The specific interaction was identified by the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE) with the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Techniques Used: Transformation Assay, Plasmid Preparation, Activation Assay, Negative Control, Positive Control

    Determination of the YFV NS5 domain responsible for the interaction with eIF3L. The figure shows a representation of RNApol NS5 YFV and its fragments, with deletions (1–14) used to map the interaction domain with the eIF3L protein in the yeast two-hybrid system. The yeast was co-transformed with the pGBKT7-deletion constructs and pACT2-eIF3L. The interaction occurs between amino acids 368 and 448 (interaction domain, the region in black in the schematic representation), which was identified by the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE) with the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.
    Figure Legend Snippet: Determination of the YFV NS5 domain responsible for the interaction with eIF3L. The figure shows a representation of RNApol NS5 YFV and its fragments, with deletions (1–14) used to map the interaction domain with the eIF3L protein in the yeast two-hybrid system. The yeast was co-transformed with the pGBKT7-deletion constructs and pACT2-eIF3L. The interaction occurs between amino acids 368 and 448 (interaction domain, the region in black in the schematic representation), which was identified by the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE) with the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Techniques Used: Transformation Assay, Construct, Activation Assay, Negative Control, Positive Control

    The eIF3L protein specifically interacts with the N - terminal region of the NS5 YFV interaction domain. The yeast was co-transformed with the pGBKT7 segments and pACT2-eIF3L (or the empty or large T-antigen SV40 AD vector). Segment 3 NS5 (ID) and eIF3L interaction was detected by activation of the reporter genes HIS 3 and ADE 2, which led to the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE). C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.
    Figure Legend Snippet: The eIF3L protein specifically interacts with the N - terminal region of the NS5 YFV interaction domain. The yeast was co-transformed with the pGBKT7 segments and pACT2-eIF3L (or the empty or large T-antigen SV40 AD vector). Segment 3 NS5 (ID) and eIF3L interaction was detected by activation of the reporter genes HIS 3 and ADE 2, which led to the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE). C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Techniques Used: Transformation Assay, Plasmid Preparation, Activation Assay, Negative Control, Positive Control

    The mapping of the terminal region residues of the interaction domain using the yeast two - hybrid system indicates a critical interaction with eIF3L. The yeast was co-transformed with the mutant pGBKT7 constructs and pACT2-eIF3L. A positive interaction is shown by the activation of the reporter gene HIS3 in the interaction of eIF3L with the mutants ID (D436N), ID (D436S), and ID (R439A/H442A), indicating that the mutations are not critical for the interaction. A negative interaction between mutant ID (F431A/W432A/V435A) and eIF3L is indicated by the absence of growth on plates lacking histidine (−His). C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.
    Figure Legend Snippet: The mapping of the terminal region residues of the interaction domain using the yeast two - hybrid system indicates a critical interaction with eIF3L. The yeast was co-transformed with the mutant pGBKT7 constructs and pACT2-eIF3L. A positive interaction is shown by the activation of the reporter gene HIS3 in the interaction of eIF3L with the mutants ID (D436N), ID (D436S), and ID (R439A/H442A), indicating that the mutations are not critical for the interaction. A negative interaction between mutant ID (F431A/W432A/V435A) and eIF3L is indicated by the absence of growth on plates lacking histidine (−His). C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Techniques Used: Transformation Assay, Mutagenesis, Construct, Activation Assay, Negative Control, Positive Control

    The interaction domains of other flaviviruses interact with eIF3L. The yeast cells were co-transformed with pGBKT7-ID ( Flavivirus members dengue virus types 3 and 4 and St. Louis encephalitis) and pACT2-eIF3L (or the empty or large T-antigen SV40 AD vector). The interaction domains of other flaviviruses (DENV types 3 and 4 and SLE) present a positive interaction with the eIF3L protein through the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.
    Figure Legend Snippet: The interaction domains of other flaviviruses interact with eIF3L. The yeast cells were co-transformed with pGBKT7-ID ( Flavivirus members dengue virus types 3 and 4 and St. Louis encephalitis) and pACT2-eIF3L (or the empty or large T-antigen SV40 AD vector). The interaction domains of other flaviviruses (DENV types 3 and 4 and SLE) present a positive interaction with the eIF3L protein through the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Techniques Used: Transformation Assay, Plasmid Preparation, Activation Assay, Negative Control, Positive Control

    Related Articles

    Clone Assay:

    Article Title: HM1.24 Is Internalized from Lipid Rafts by Clathrin-mediated Endocytosis through Interaction with ?-Adaptin
    Article Snippet: Yeast Two-hybrid Assays The MATCHMAKER Two-Hybrid 3 (Clontech) was used. .. The cytoplasmic tail of HM1.24 corresponding to amino acid residues 1–21 was cloned into pGBKT7, and the plasmid was transformed into the yeast strain AH109 followed by screening a pACT2 HeLa cell cDNA library (Clontech). ..

    Plasmid Preparation:

    Article Title: HM1.24 Is Internalized from Lipid Rafts by Clathrin-mediated Endocytosis through Interaction with ?-Adaptin
    Article Snippet: Yeast Two-hybrid Assays The MATCHMAKER Two-Hybrid 3 (Clontech) was used. .. The cytoplasmic tail of HM1.24 corresponding to amino acid residues 1–21 was cloned into pGBKT7, and the plasmid was transformed into the yeast strain AH109 followed by screening a pACT2 HeLa cell cDNA library (Clontech). ..

    Article Title: Adapter protein NRBP associates with Jab1 and negatively regulates AP-1 activity.
    Article Snippet: A yeast two-hybrid screen was performed using the Matchmaker GAL4 Two-Hybrid System 3 (Clontech). .. Full-length human NRBP cDNA was subcloned into pGBKT7 vector and the resulting bait plasmid was used to screen a pACT2 HeLa cell cDNA library (Clontech) following the manufacturer’s protocol. ..

    Article Title: The eukaryotic translation initiation factor 3 subunit L protein interacts with Flavivirus NS5 and may modulate yellow fever virus replication
    Article Snippet: The yeast strain Saccharomyces cerevisiae AH109 (BD MATCHMAKER GAL4 Two-Hybrid System 3, Clontech, USA) was used for the two-hybrid screen. .. The bait plasmid pGBKT7-RNApol and the pACT2 HeLa cell cDNA library (Clontech, USA) were sequentially introduced into the yeast cells using the EZ-Yeast Transformation kit - Single Vector Transformation protocol (Q.BIO gene, USA). ..

    Article Title: The eukaryotic translation initiation factor 3 subunit L protein interacts with FlavivirusNS5 and may modulate yellow fever virus replication
    Article Snippet: The yeast strain Saccharomyces cerevisiae AH109 (BD MATCHMAKER GAL4 Two-Hybrid System 3, Clontech, USA) was used for the two-hybrid screen. .. The bait plasmid pGBKT7-RNApol and the pACT2 HeLa cell cDNA library (Clontech, USA) were sequentially introduced into the yeast cells using the EZ-Yeast Transformation kit - Single Vector Transformation protocol (Q.BIO gene, USA). ..

    Transformation Assay:

    Article Title: HM1.24 Is Internalized from Lipid Rafts by Clathrin-mediated Endocytosis through Interaction with ?-Adaptin
    Article Snippet: Yeast Two-hybrid Assays The MATCHMAKER Two-Hybrid 3 (Clontech) was used. .. The cytoplasmic tail of HM1.24 corresponding to amino acid residues 1–21 was cloned into pGBKT7, and the plasmid was transformed into the yeast strain AH109 followed by screening a pACT2 HeLa cell cDNA library (Clontech). ..

    Article Title: The eukaryotic translation initiation factor 3 subunit L protein interacts with Flavivirus NS5 and may modulate yellow fever virus replication
    Article Snippet: The yeast strain Saccharomyces cerevisiae AH109 (BD MATCHMAKER GAL4 Two-Hybrid System 3, Clontech, USA) was used for the two-hybrid screen. .. The bait plasmid pGBKT7-RNApol and the pACT2 HeLa cell cDNA library (Clontech, USA) were sequentially introduced into the yeast cells using the EZ-Yeast Transformation kit - Single Vector Transformation protocol (Q.BIO gene, USA). ..

    Article Title: The eukaryotic translation initiation factor 3 subunit L protein interacts with FlavivirusNS5 and may modulate yellow fever virus replication
    Article Snippet: The yeast strain Saccharomyces cerevisiae AH109 (BD MATCHMAKER GAL4 Two-Hybrid System 3, Clontech, USA) was used for the two-hybrid screen. .. The bait plasmid pGBKT7-RNApol and the pACT2 HeLa cell cDNA library (Clontech, USA) were sequentially introduced into the yeast cells using the EZ-Yeast Transformation kit - Single Vector Transformation protocol (Q.BIO gene, USA). ..

    cDNA Library Assay:

    Article Title: HM1.24 Is Internalized from Lipid Rafts by Clathrin-mediated Endocytosis through Interaction with ?-Adaptin
    Article Snippet: Yeast Two-hybrid Assays The MATCHMAKER Two-Hybrid 3 (Clontech) was used. .. The cytoplasmic tail of HM1.24 corresponding to amino acid residues 1–21 was cloned into pGBKT7, and the plasmid was transformed into the yeast strain AH109 followed by screening a pACT2 HeLa cell cDNA library (Clontech). ..

    Article Title: Adapter protein NRBP associates with Jab1 and negatively regulates AP-1 activity.
    Article Snippet: A yeast two-hybrid screen was performed using the Matchmaker GAL4 Two-Hybrid System 3 (Clontech). .. Full-length human NRBP cDNA was subcloned into pGBKT7 vector and the resulting bait plasmid was used to screen a pACT2 HeLa cell cDNA library (Clontech) following the manufacturer’s protocol. ..

    Article Title: The eukaryotic translation initiation factor 3 subunit L protein interacts with Flavivirus NS5 and may modulate yellow fever virus replication
    Article Snippet: The yeast strain Saccharomyces cerevisiae AH109 (BD MATCHMAKER GAL4 Two-Hybrid System 3, Clontech, USA) was used for the two-hybrid screen. .. The bait plasmid pGBKT7-RNApol and the pACT2 HeLa cell cDNA library (Clontech, USA) were sequentially introduced into the yeast cells using the EZ-Yeast Transformation kit - Single Vector Transformation protocol (Q.BIO gene, USA). ..

    Article Title: The eukaryotic translation initiation factor 3 subunit L protein interacts with FlavivirusNS5 and may modulate yellow fever virus replication
    Article Snippet: The yeast strain Saccharomyces cerevisiae AH109 (BD MATCHMAKER GAL4 Two-Hybrid System 3, Clontech, USA) was used for the two-hybrid screen. .. The bait plasmid pGBKT7-RNApol and the pACT2 HeLa cell cDNA library (Clontech, USA) were sequentially introduced into the yeast cells using the EZ-Yeast Transformation kit - Single Vector Transformation protocol (Q.BIO gene, USA). ..



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    pact2 hela cell cdna library  (TaKaRa)
    93
    TaKaRa pact2 hela cell cdna library
    YFV NS5 (RNApol)- human eIF3L interaction in a yeast two - hybrid system. Yeast strain AH109 was co-transformed with pGBKT7-RNApol (or the empty or Lamin C human protein BD vector) and <t>pACT2-eIF3L</t> (or the empty or SV40 large T-antigen AD vector). The specific interaction was identified by the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE) with the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.
    Pact2 Hela Cell Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pact2+hela+cell+cdna+library/Human+Kidney+Matchmaker+cDNA+Library+pACT2/pmc03698205-138-6-11
    Average 93 stars, based on 1 article reviews
    pact2 hela cell cdna library - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    YFV NS5 (RNApol)- human eIF3L interaction in a yeast two - hybrid system. Yeast strain AH109 was co-transformed with pGBKT7-RNApol (or the empty or Lamin C human protein BD vector) and pACT2-eIF3L (or the empty or SV40 large T-antigen AD vector). The specific interaction was identified by the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE) with the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Journal: Virology Journal

    Article Title: The eukaryotic translation initiation factor 3 subunit L protein interacts with Flavivirus NS5 and may modulate yellow fever virus replication

    doi: 10.1186/1743-422X-10-205

    Figure Lengend Snippet: YFV NS5 (RNApol)- human eIF3L interaction in a yeast two - hybrid system. Yeast strain AH109 was co-transformed with pGBKT7-RNApol (or the empty or Lamin C human protein BD vector) and pACT2-eIF3L (or the empty or SV40 large T-antigen AD vector). The specific interaction was identified by the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE) with the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Article Snippet: The bait plasmid pGBKT7-RNApol and the pACT2 HeLa cell cDNA library (Clontech, USA) were sequentially introduced into the yeast cells using the EZ-Yeast Transformation kit - Single Vector Transformation protocol (Q.BIO gene, USA).

    Techniques: Transformation Assay, Plasmid Preparation, Activation Assay, Negative Control, Positive Control

    Determination of the YFV NS5 domain responsible for the interaction with eIF3L. The figure shows a representation of RNApol NS5 YFV and its fragments, with deletions (1–14) used to map the interaction domain with the eIF3L protein in the yeast two-hybrid system. The yeast was co-transformed with the pGBKT7-deletion constructs and pACT2-eIF3L. The interaction occurs between amino acids 368 and 448 (interaction domain, the region in black in the schematic representation), which was identified by the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE) with the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Journal: Virology Journal

    Article Title: The eukaryotic translation initiation factor 3 subunit L protein interacts with Flavivirus NS5 and may modulate yellow fever virus replication

    doi: 10.1186/1743-422X-10-205

    Figure Lengend Snippet: Determination of the YFV NS5 domain responsible for the interaction with eIF3L. The figure shows a representation of RNApol NS5 YFV and its fragments, with deletions (1–14) used to map the interaction domain with the eIF3L protein in the yeast two-hybrid system. The yeast was co-transformed with the pGBKT7-deletion constructs and pACT2-eIF3L. The interaction occurs between amino acids 368 and 448 (interaction domain, the region in black in the schematic representation), which was identified by the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE) with the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Article Snippet: The bait plasmid pGBKT7-RNApol and the pACT2 HeLa cell cDNA library (Clontech, USA) were sequentially introduced into the yeast cells using the EZ-Yeast Transformation kit - Single Vector Transformation protocol (Q.BIO gene, USA).

    Techniques: Transformation Assay, Construct, Activation Assay, Negative Control, Positive Control

    The eIF3L protein specifically interacts with the N - terminal region of the NS5 YFV interaction domain. The yeast was co-transformed with the pGBKT7 segments and pACT2-eIF3L (or the empty or large T-antigen SV40 AD vector). Segment 3 NS5 (ID) and eIF3L interaction was detected by activation of the reporter genes HIS 3 and ADE 2, which led to the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE). C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Journal: Virology Journal

    Article Title: The eukaryotic translation initiation factor 3 subunit L protein interacts with Flavivirus NS5 and may modulate yellow fever virus replication

    doi: 10.1186/1743-422X-10-205

    Figure Lengend Snippet: The eIF3L protein specifically interacts with the N - terminal region of the NS5 YFV interaction domain. The yeast was co-transformed with the pGBKT7 segments and pACT2-eIF3L (or the empty or large T-antigen SV40 AD vector). Segment 3 NS5 (ID) and eIF3L interaction was detected by activation of the reporter genes HIS 3 and ADE 2, which led to the growth of transformants on SD medium (−LEU, -TRP, -HIS) and SD medium (−LEU, -TRP, -HIS, -ADE). C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Article Snippet: The bait plasmid pGBKT7-RNApol and the pACT2 HeLa cell cDNA library (Clontech, USA) were sequentially introduced into the yeast cells using the EZ-Yeast Transformation kit - Single Vector Transformation protocol (Q.BIO gene, USA).

    Techniques: Transformation Assay, Plasmid Preparation, Activation Assay, Negative Control, Positive Control

    The mapping of the terminal region residues of the interaction domain using the yeast two - hybrid system indicates a critical interaction with eIF3L. The yeast was co-transformed with the mutant pGBKT7 constructs and pACT2-eIF3L. A positive interaction is shown by the activation of the reporter gene HIS3 in the interaction of eIF3L with the mutants ID (D436N), ID (D436S), and ID (R439A/H442A), indicating that the mutations are not critical for the interaction. A negative interaction between mutant ID (F431A/W432A/V435A) and eIF3L is indicated by the absence of growth on plates lacking histidine (−His). C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Journal: Virology Journal

    Article Title: The eukaryotic translation initiation factor 3 subunit L protein interacts with Flavivirus NS5 and may modulate yellow fever virus replication

    doi: 10.1186/1743-422X-10-205

    Figure Lengend Snippet: The mapping of the terminal region residues of the interaction domain using the yeast two - hybrid system indicates a critical interaction with eIF3L. The yeast was co-transformed with the mutant pGBKT7 constructs and pACT2-eIF3L. A positive interaction is shown by the activation of the reporter gene HIS3 in the interaction of eIF3L with the mutants ID (D436N), ID (D436S), and ID (R439A/H442A), indicating that the mutations are not critical for the interaction. A negative interaction between mutant ID (F431A/W432A/V435A) and eIF3L is indicated by the absence of growth on plates lacking histidine (−His). C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Article Snippet: The bait plasmid pGBKT7-RNApol and the pACT2 HeLa cell cDNA library (Clontech, USA) were sequentially introduced into the yeast cells using the EZ-Yeast Transformation kit - Single Vector Transformation protocol (Q.BIO gene, USA).

    Techniques: Transformation Assay, Mutagenesis, Construct, Activation Assay, Negative Control, Positive Control

    The interaction domains of other flaviviruses interact with eIF3L. The yeast cells were co-transformed with pGBKT7-ID ( Flavivirus members dengue virus types 3 and 4 and St. Louis encephalitis) and pACT2-eIF3L (or the empty or large T-antigen SV40 AD vector). The interaction domains of other flaviviruses (DENV types 3 and 4 and SLE) present a positive interaction with the eIF3L protein through the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Journal: Virology Journal

    Article Title: The eukaryotic translation initiation factor 3 subunit L protein interacts with Flavivirus NS5 and may modulate yellow fever virus replication

    doi: 10.1186/1743-422X-10-205

    Figure Lengend Snippet: The interaction domains of other flaviviruses interact with eIF3L. The yeast cells were co-transformed with pGBKT7-ID ( Flavivirus members dengue virus types 3 and 4 and St. Louis encephalitis) and pACT2-eIF3L (or the empty or large T-antigen SV40 AD vector). The interaction domains of other flaviviruses (DENV types 3 and 4 and SLE) present a positive interaction with the eIF3L protein through the activation of the reporter genes HIS 3 and ADE 2. C-, negative control; C+, positive control; N, normalized original culture sample; 1–4, 10-fold serial dilutions of sample.

    Article Snippet: The bait plasmid pGBKT7-RNApol and the pACT2 HeLa cell cDNA library (Clontech, USA) were sequentially introduced into the yeast cells using the EZ-Yeast Transformation kit - Single Vector Transformation protocol (Q.BIO gene, USA).

    Techniques: Transformation Assay, Plasmid Preparation, Activation Assay, Negative Control, Positive Control